Twenty Division-I American Football athletes (age = 19.1 ± 1.1 y) participated in a 6-week off-season strength and conditioning program. Athletes resistance trained for 6 weeks at 85-100% of their 1-repetition maximum (RM). Evaluations were performed at Week 1, Week 4, and Week 6 of training. At Week 6, resting measures of both IL-6 and cortisol were elevated above Week 1 baseline measures (p<0.05). Body weight increased Weeks 2-5 as well as 1-RM on the three main lifts investigated (Bench = +4.8 ± 4.2%; Squat = +2.1 ± 3.1%; Clean = +2.0 ± 3.3%). REST-Q questionnaire showed small, significant decreases in four perceived affective categories. Correlation coefficients revealed significant relationships of IL-6 and cortisol at Weeks 1 and Week 6. It appears that the training utilized in the study was strenuous enough to produce a positive physical response and increases in biomarkers, but did not cause overtraining.