The search for targeting molecules that allow therapies to be site-directed and specific is a very important area of research. Small engineered protein domains are well suited because of their size to be utilized as such targeting molecules. This thesis aims to examine the process of directed forward evolution of a small three helix bundle domain (3HB) library. mRNA display pre-selection was performed to analyze the 3HB library's potential to be successful in that process. 3HB genes were shown to be expressed at very low levels in E.Coli. Also, sequencing analysis from a pGFP-3HB fusion library is presented.