EVALUATION OF EXPOSURE TO ACETAMIPRID IN PESTICIDE APPLICATORS
Public Deposited- Last Modified
- February 28, 2019
- Creator
-
Salsbury, Mark
- Affiliation: Gillings School of Global Public Health, Department of Environmental Sciences and Engineering
- Abstract
- Acetamiprid is one of the newest and most frequently applied insecticides in agricultural settings due to low mammalian toxicity and effectiveness in strains of insects that have developed resistance to traditional insecticides. Pesticide workers who mix, handle, and apply acetamiprid-containing pesticides typically receive the largest dose through the dermal exposure route. Reliable and sensitive methods are needed to evaluate dermal exposure to acetamiprid in pesticide workers. This study was conducted to develop and apply a tape-strip method to quantitatively measure dermal exposure to acetamiprid. In addition, we explored the suitability of using either specific-gravity or creatinine normalized urine acetamiprid concentration as a biomarker of dermal exposure. Five part-time pesticide applicators were monitored using tape-strip sampling of the skin and collecting pre- and post-exposure urine samples during a spray application of acetamiprid-containing pesticide in Honduras. A liquid-chromatography tandem-mass-spectrometry method was used to quantify acetamiprid in tape-strip and urine samples (limit of quantitation for tape 10 fmol/μL and for urine sample 67 fmol/μL). Acetamiprid was detected in all tape-strip samples, with the greatest exposures measured on the arms (mean left arm 37.0 μg, mean right arm 37.4 μg) followed by the left hand (mean 27.7 μg). The estimated dermal dose was calculated for each applicator based on the tape-strip measurements and the area of skin exposed during pesticide application. A positive correlation (R^2=0.66, P=0.097) was observed between the estimated dermal dose and specific-gravity normalized acetamiprid concentrations in urine. Specific-gravity normalization of spot urine samples appears to be more suitable than creatinine normalization in biomarker studies of exposure to acetamiprid . The results of this study indicate that tape-stripping provides a suitable method to evaluate dermal exposure to acetamiprid-containing pesticides. Further studies with larger sample sizes are warranted to ascertain the relationship between dermal exposure and specific-gravity or creatinine normalized acetamiprid levels in urine.
- Date of publication
- August 2011
- DOI
- Resource type
- Rights statement
- In Copyright
- Advisor
- Bodnar, Wanda
- Nylander-French, Leena A.
- Flynn, Michael
- Degree
- Master of Science in Public Health
- Academic concentration
- Environmental Health Sciences
- Degree granting institution
- University of North Carolina at Chapel Hill
- Graduation year
- 2011
- Language
- Deposit record
- e00f1df6-6c31-41ba-867b-150d86a8c8cb
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