Porphyromonas gingivalis oral infection exacerbates the development and severity of collagen-induced arthritis
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Marchesan, Julie Teresa, et al. Porphyromonas Gingivalis Oral Infection Exacerbates the Development and Severity of Collagen-induced Arthritis. BioMed Central Ltd, 2013. https://doi.org/10.17615/ke5g-gm19APA
Marchesan, J., Gerow, E., Schaff, R., Taut, A., Shin, S., Sugai, J., Brand, D., Burberry, A., Jorns, J., Lundy, S., Nuñez, G., Fox, D., & Giannobile, W. (2013). Porphyromonas gingivalis oral infection exacerbates the development and severity of collagen-induced arthritis. BioMed Central Ltd. https://doi.org/10.17615/ke5g-gm19Chicago
Marchesan, Julie Teresa, Elizabeth Ann Gerow, Riley Schaff, Andrei Dan Taut, Seung Yun Shin, James Sugai, David Brand et al. 2013. Porphyromonas Gingivalis Oral Infection Exacerbates the Development and Severity of Collagen-Induced Arthritis. BioMed Central Ltd. https://doi.org/10.17615/ke5g-gm19- Creator
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Marchesan, Julie Teresa
- Other Affiliation: Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI, USA
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Gerow, Elizabeth Ann
- Other Affiliation: Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI, USA
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Schaff, Riley
- Other Affiliation: Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI, USA
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Taut, Andrei Dan
- Other Affiliation: Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI, USA
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Shin, Seung-Yun
- Other Affiliation: Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI, USA; Department of Periodontology, Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, South Korea
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Sugai, James
- Other Affiliation: Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI, USA
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Brand, David
- Other Affiliation: Research Service, Veterans Affairs Medical Center, Memphis, TN, USA
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Burberry, Aaron
- Other Affiliation: Department of Pathology, University of Michigan Medical School, Ann Arbor, MI, USA
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Jorns, Julie
- Other Affiliation: Department of Pathology, University of Michigan Medical School, Ann Arbor, MI, USA
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Lundy, Steven Karl
- Other Affiliation: Department of Internal Medicine – Division of Rheumatology, University of Michigan Medical School, Ann Arbor, MI, USA
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Nuñez, Gabriel
- Other Affiliation: Department of Pathology, University of Michigan Medical School, Ann Arbor, MI, USA
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Fox, David A
- Other Affiliation: Department of Internal Medicine – Division of Rheumatology, University of Michigan Medical School, Ann Arbor, MI, USA
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Giannobile, William V
- Other Affiliation: Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI, USA
- Abstract
- Abstract Introduction: Clinical studies suggest a direct influence of periodontal disease (PD) on serum inflammatory markers and disease assessment of patients with established rheumatoid arthritis (RA). However, the influence of PD on arthritis development remains unclear. This investigation was undertaken to determine the contribution of chronic PD to immune activation and development of joint inflammation using the collagen-induced arthritis (CIA) model. Methods: DBA1/J mice orally infected with Porphyromonas gingivalis were administered with collagen II (CII) emulsified in complete Freund’s adjuvant (CFA) or incomplete Freund’s adjuvant (IFA) to induce arthritis. Arthritis development was assessed by visual scoring of paw swelling, caliper measurement of the paws, mRNA expression, paw micro-computed tomography (micro-CT) analysis, histology, and tartrate resistant acid phosphatase for osteoclast detection (TRAP)-positive immunohistochemistry. Serum and reactivated splenocytes were evaluated for cytokine expression. Results: Mice induced for PD and/or arthritis developed periodontal disease, shown by decreased alveolar bone and alteration of mRNA expression in gingival tissues and submandibular lymph nodes compared to vehicle. P. gingivalis oral infection increased paw swelling and osteoclast numbers in mice immunized with CFA/CII. Arthritis incidence and severity were increased by P. gingivalis in mice that received IFA/CII immunizations. Increased synovitis, bone erosions, and osteoclast numbers in the paws were observed following IFA/CII immunizations in mice infected with P gingivalis. Furthermore, cytokine analysis showed a trend toward increased serum Th17/Th1 ratios when P. gingivalis infection was present in mice receiving either CFA/CII or IFA/CII immunizations. Significant cytokine increases induced by P. gingivalis oral infection were mostly associated to Th17-related cytokines of reactivated splenic cells, including IL-1β, IL-6, and IL-22 in the CFA/CII group and IL-1β, tumor necrosis factor-α, transforming growth factor-β, IL-6 and IL-23 in the IFA/CII group. Conclusions: Chronic P. gingivalis oral infection prior to arthritis induction increases the immune system activation favoring Th17 cell responses, and ultimately accelerating arthritis development. These results suggest that chronic oral infection may influence RA development mainly through activation of Th17-related pathways.
- Date of publication
- November 12, 2013
- DOI
- Identifier
- Resource type
- Article
- Rights statement
- In Copyright
- Rights holder
- Julie Teresa Marchesan et al.; licensee BioMed Central Ltd.
- License
- Journal title
- Arthritis Research & Therapy
- Journal volume
- 15
- Journal issue
- 6
- Page start
- R186
- Language
- English
- Is the article or chapter peer-reviewed?
- Yes
- ISSN
- 1478-6354
- Bibliographic citation
- Arthritis Research & Therapy. 2013 Nov 12;15(6):R186
- Publisher
- BioMed Central Ltd
- Access right
- Open Access
- Date uploaded
- December 12, 2013
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