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Figure 3 TRIB3 colocalizes with pimonidazole in human breast cancer tissue. Results after linear unmixing of scanned images from immunohistochemical stainings of (a) TRIB3 (DAB, brown) and (b) pimonidazole (DAB, brown), and single images from immunohistochemical stainings at 100× magnification of (c) TRIB3 (DAB, brown), (d) pimonidazole (DAB, brown) and (e) hematoxylin (blue), in human breast cancer tissue. See methods for details of unmixing and scanning.