Evaluation of different microporous filters for the recovery of human adeno- and noroviruses from seawater
Public Deposited- Last Modified
- February 28, 2019
- Creator
-
Lyman, Whitney
- Affiliation: Gillings School of Global Public Health, Department of Environmental Sciences and Engineering
- Abstract
- The presence of bacterial enteric pathogens or their indicators does not predict the presence of human enteric viruses in recreational waters. Currently, no validated filter-based method exists for the recovery and quantitative detection of human noro- or adenovirus from seawater. In this study, we evaluated the adsorption-elution characteristics of two negatively charged fiberglass filters (Whatman GF/F and Filterite) and a novel positively charged alumina filter (NanoCeram) for the recovery of noro- and adenovirus in seeded seawater. Percent adsorption and elution were calculated by realtime (RT-)PCR detection of the viruses in the filter eluates. Different elution solutions and contact times were compared for subsequent recovery of the adsorbed viruses. The negatively charged filters had both low adsorption of adenovirus type 2 from seawater (GF/F:26%; Filterite:11%) and low elution of the viruses from the filters (<10%). The positively charged Nanoceram filters adsorbed significantly more virus (>58%) than the negatively charged filters, but effective elution of the viruses from these filters was very poor (<5%) regardless of the type of elution buffer. Further research into improved elution methods or different filters for the recovery of these viral pathogens from seawater is warranted to better monitor health risks associated with exposure to contaminated recreational waters.
- Date of publication
- August 2005
- DOI
- Resource type
- Rights statement
- In Copyright
- Advisor
- DiGiano, Francis A.
- Sobsey, Mark
- Vinjé, Jan
- Degree
- Master of Science in Public Health
- Academic concentration
- Environmental Health Sciences
- Degree granting institution
- University of North Carolina at Chapel Hill
- Graduation year
- 2005
- Language
- Deposit record
- 8daa46ae-58bd-4ddf-8097-0c44062f7d48
Relations
- Parents:
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