Application of InVitro Cell Culture Assay to Cryptosporidium Parvum Oocysts Inactive by Thermal Processes
Public Deposited- Last Modified
- February 28, 2019
- Creator
-
Barbee, Susan Lynn
- Affiliation: Gillings School of Global Public Health, Department of Environmental Sciences and Engineering
- Abstract
- Cryptosporidium is an important waterborne pathogen and its oocysts are very persistent in the environment and very resistant to chemical disinfection. Inactivation of Cryptosporidium parvum oocysts by heat is a potential disinfection process for water, wastewater and biosolids. We studied the kinetics and extent of thermal inactivation of C. parvum oocysts in buffered water at 35°, 49°, 50°, 55° and 60°C, and by thermophilic anaerobic digestion of cow manure at 55°C using mammalian cell culture to quantify oocysts infectivity. Comparisons of infectious oocyst concentrations between untreated controls and thermally treated samples indicated significant losses of oocyst infectivity by higher temperature thermal treatment in both milieu. While there were only minor reductions of oocyst infectivity in buffered water after 24 hours at 23° and 35°C, temperatures of 55° and 60°C reduced oocyst infectivity by 99.9% within 60 minutes. Exposure at 55°C in anaerobically digested manure for 60 minutes reduced oocyst infectivity by 99.9%, but in the untreated manure oocyst infectivity was stable for many hours at 23°C. These data demonstrate that the infectivity of C. parvum oocysts is rapidly and extensively reduced in water and bovine manure at thermophilic temperatures as low as 55°C.
- Date of publication
- May 1998
- DOI
- Resource type
- Rights statement
- In Copyright
- Advisor
- Sobsey, Mark
- Aitken, Michael
- Seed, J. Richard
- Degree
- Master of Science in Public Health
- Academic concentration
- Environmental Health Sciences
- Degree granting institution
- University of North Carolina at Chapel Hill
- Graduation year
- 1998
- Language
- Deposit record
- 56f7e058-1c97-4774-9946-7afdcd8e0e36
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