Expression of Fatty Acid Oxidation-Related Genes in Acsl4 L -/- Primary Hepatocytes

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  • February 26, 2019
Creator
  • Lin, Dennis
    • Affiliation: Gillings School of Global Public Health, Department of Nutrition
Abstract
  • Long-chain acyl-CoA synthetases are a family of enzymes responsible for catalyzing the reaction converting long chain fatty acids to fatty acyl-CoA. ACSL4, a specific member of this family, plays a role in eicosanoid metabolism and has been found to be associated with liver diseases including non-alcoholic fatty liver disease and hepatocellular carcinoma. In order to study ACSL4 in the context of hepatic lipid metabolism, a murine liver-specific Acsl4 knockout (Acsl4 L -/-) was developed and characterized in our lab. Based on a previous experiment in our lab, we hypothesized that Acsl4 L -/- would cause a defect in beta adrenergic signaling. In order to test this hypothesis, we conducted experiments to determine the effects of various treatments on the expression of fatty acid oxidation-related genes in both knockout and control hepatocytes. Treatments with fatty acids caused increases in expression of several fatty acid oxidation-related genes in both knockout and control hepatocytes. Hormone treatments did not cause anticipated increases in expression of fatty acid oxidation-related genes in several of the control groups. Because of this we were not able to make conclusions about the effects of the knockout. These results indicated that Acsl4 L -/- did not cause a defect in beta adrenergic signaling.
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Rights statement
  • In Copyright
Advisor
  • Coleman, Rosalind
Degree
  • Bachelor of Science in Public Health
Academic concentration
  • Nutrition
Honors level
  • Honors
Degree granting institution
  • University of North Carolina at Chapel Hill
Graduation year
  • 2018
Language
  • English

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