Metals as Potential Biomarkers of Exposure to Carbon Disulfide
Public Deposited- Last Modified
- February 28, 2019
- Creator
-
Parham, Alan G.
- Affiliation: Gillings School of Global Public Health, Department of Environmental Sciences and Engineering
- Abstract
- Previous studies have hypothesized that Carbon Disulfide (CS2) toxicity is based on the chelation of copper and zinc ions by the dithiocarbamate derivatives arising from the reaction of CS2 with amines. The primary objective of this study was to investigate the potential involvement of trace metals in the metabolism of CS2 in exposed rats. CS2 was administered in air at 0, 50, 150, or 500 ppm to separate groups of 60 young adult male Sprague-Dawley rats for 6 hr/day, 5 days/wk, for 6.5 months. The diet consisted of NIH 07 pellets (Ziegler Brothers, Gardner, PA) and tap water from bottles. After the rats had been exposed to CS2 for 6.5 months, exposure was discontinued. Nine rats from each exposure group including controls were chosen randomly. Cumulative voided urine was collected during 0-8, 8-16, 16-24, and 24-48 hours following the last CS2 exposure. Thirty-seven metals were measured in the urine by inductively coupled plasma atomic emission spectroscopy (ICP-AES). Linear regression analysis using creatinine normalized concentrations for each exposure group over time revealed statistically significant (p<0.05) correlation coefficients for B, Ca, I, Mg, P, Pt, and Sr in the control group; Ca, I, Mg, Mn, and P in the 50 ppm group; Se and Si in the 150 ppm group; and B, Ca, Mg, Mn, and P in the 500 ppm group. With the exception of Pt, Se, and Si, the metal concentrations were decreasing over time. Linear regression analysis using total metal (μg) per time period instead of creatinine normalized (μg/mg) concentrations, yielded similar results. Since the majority of metals with a significant correlation coefficient in both analysis were in the non-exposed group, it is unlikely that the variations were a result of CS2 exposure. Future studies should concentrate on determining the natural fluctuations of urinary excretion of trace metals in rats.
- Date of publication
- August 2001
- DOI
- Resource type
- Rights statement
- In Copyright
- Advisor
- Ball, Louise
- Symons, Michael J.
- Cox, Clint
- Degree
- Master of Public Health
- Academic concentration
- Environmental Health Sciences
- Degree granting institution
- University of North Carolina at Chapel Hill
- Graduation year
- 2001
- Language
- Deposit record
- 56f7e058-1c97-4774-9946-7afdcd8e0e36
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