The purpose of the current study was to examine the effect of aerobic endurance exercise (AEE), high-intensity interval training (HIIT), and high-intensity resistance training (HIRT), combined with pre-exercise carbohydrate (CHO) or protein (PRO) ingestion on resting energy expenditure (REE) and respiratory exchange ratio (RER) in women. Participants completed six sessions, of three exercise modalities: AEE, HIIT, and HIRT, and two acute nutritional interventions: 25 grams of CHO and PRO. Measurements were taken at baseline, immediately post, 30min post and 60 min post. Post exercise, HIIT elicited the largest increase in REE compared to AEE (p<0.0001) and HIRT (p<0.0001). HIIT and HIRT resulted in significantly lower RER (p<0.0001-p=0.0004) compared to AEE post exercise. PRO intake significantly elevated REE (p<0.01) and reduced RER (p=0.0012) to a greater extent than CHO beginning 30min post exercise. Integrating HIIT and pre-exercise PRO intake may have positive implications on energy expenditure and fat utilization in women.