Discovery and characterization of a novel antimicrobial peptide from Houttuynia cordata (chameleon plant) using PepSAVI-MS

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  • February 26, 2019
Creator
  • Wu, Xuelan
    • Affiliation: College of Arts and Sciences, Department of Chemistry
Abstract
  • While historical screening efforts on drug discovery have largely focused on small molecule constituents of natural products, the antimicrobial drugs made from these small molecules have cultivated resistance in many microorganisms. Recent studies have shown that ribosomally-synthesized, post-translationally-modified peptide natural products (RiPPs) have the potential to address this urgent need for new therapeutic compounds due to their target specificity, high potency and metabolic stability. While these bioactive RiPPs are ubiquitously expressed in natural products, plants are a promising and largely unexplored source for these peptides. To expedite the search for novel bioactive peptides, the PepSAVI-MS pipeline was developed in the Hicks Lab. This pipeline begins with zero knowledge about the peptide profile of the species, but employs mass spectrometry and statistics to quickly determine the most-likely bioactive peptide targets. In this paper, Houttuynia cordata (chameleon plant) is subjected to PepSAVI-MS. Our data showed that fractions of its peptide library exhibit strong bioactivity against Escherichia coli, as well as the multi-drug resistant pathogens Acinetobacter baumannii, Klebsiella pneumoniae, and Staphylococcus aureus (gram-positive). The putative antimicrobial peptide candidates were identified using a hybrid LC-MS/MS and statistical analysis-based approach. This paper will focus on the discovery and characterization of the peptide candidate that has the highest probability to possess antimicrobial property.
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  • In Copyright
Note
  • Funding: None
Advisor
  • Hicks, Leslie
Degree
  • Bachelor of Science
Academic concentration
  • Chemistry
Honors level
  • Honors
Degree granting institution
  • University of North Carolina at Chapel Hill
Graduation year
  • 2017
Language
  • English

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