Indentification of the Metabolites of 3-Nitrofluoranthene and 2-Nitrofluoranthene Formed by Samonella Typhimurium Strains TA98, TA98 ND, and TA98/1, 8-DNP6
Public Deposited- Last Modified
- February 28, 2019
- Creator
-
Stocking, Lisa M.
- Affiliation: Gillings School of Global Public Health, Department of Environmental Sciences and Engineering
- Abstract
- 3-Nitrofluoranthene (3-NFA) and 2-nitrofluoranthene (2-NFA) are environmental pollutants produced during combustion processes, proven to be mutagenic in bacterial assays and potentially carcinogenic in mammalian species. 3-NFA was incubated with Salmonella typhimurium strain TA98 and its variants TA98 ND and TA98/1,8-DNP6, deficient in nitroreductase and transacetylase enzymes respectively. All three strains produced equal amounts of the reductive metabolite 3-aminofluoranthene (3-AFA) over six hours of incubation. The nitroreductase deficient strain TA98 ND produced the metabolite at a slower initial rate of conversion than the nitroreductase proficient TA98 and TA98/1,8-DNP6. 2-NFA incubated with TA98 and TA98 ND produced two identifiable metabolites 2-aminofluoranthene (2-AFA), and N-acetyl-2-aminofluoranthene (2-NAAFA) within 24 hours of incubation. The production of metabolites by TA98 ND was significantly slower than that of TA98. TA98/1,8-DNP5 produced only 2-AFA at slower rates than TA98. The metabolism of 2-NFA by the Salmonella strains progressed at slower rates than that of 3-NFA. This reflects the difference observed in the mutagenicity of these compounds as measured by the Ames assay (3-NFA highly mutagenic, 2-NFA slightly to moderately mutagenic).
- Date of publication
- January 1989
- DOI
- Resource type
- Rights statement
- In Copyright
- Advisor
- Gold, Avram
- Turner, Alvis G.
- Ball, Louise
- Degree
- Master of Science in Public Health
- Academic concentration
- Environmental Health Sciences
- Degree granting institution
- University of North Carolina at Chapel Hill
- Graduation year
- 1989
- Language
- Deposit record
- ad7e9272-7c4f-43e0-afeb-7a5f2526ad76
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