The Optimization of Microraft Production and Use for Cell Identification and Sorting
Public Deposited- Last Modified
- February 26, 2019
- Creator
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Hinson, Robert
- Affiliation: School of Medicine, UNC/NCSU Joint Department of Biomedical Engineering
- Abstract
- Microwell arrays containing magnetic microrafts provide an alternative to fluorescence-activated cell sorting (FACS) that allows for the sorting of cells using temporal characteristics and improved viability for adherent cell lines. Making these arrays from thicker layers of polydimethylsiloxane (PDMS) improved the imaging quality by reducing the sagging of the array caused by the weight of cell culture media and did not hinder the effectiveness of releasing the rafts for cell sorting. It was also found that centrifugation of cells in these arrays drastically reduced the time it took for the cells to sediment into the wells at the desired density and did not reduce the viability of the cells.
- Date of publication
- spring 2014
- Keyword
- DOI
- Resource type
- Rights statement
- In Copyright
- Note
- Funding: Tom and Elizabeth Long Excellence Fund for Honors
- Funding: UNC Summer Undergraduate Research Fellowship
- Advisor
- Allbritton, Nancy
- Degree
- Bachelor of Science
- Honors level
- Highest Honors
- Degree granting institution
- University of North Carolina at Chapel Hill
- Extent
- 10
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