α-Actinin: Immunofluorescent localization of a muscle structural protein in nonmuscle cells
Public Deposited- Creator
-
Lazarides, Elias
- Other Affiliation: Cold Spring Harbor Laboratory Cold Spring Harbor
-
Burridge, Keith
- Other Affiliation: MRC Laboratory of Molecular Biology
- Abstract
- Antibodies specific for the skeletal muscle structural protein α-actinin are used to localize this protein by indirect immunofluorescence in nonmuscle cells. In cultured nonmuscle cells, α-actinin is localized along or between actin filament bundles producing an almost regular periodicity. The protein is also detected in the form of fluorescent plaques at some ends of actin filament bundles, as well as in a filamentous form in some overlap areas of cells. In spreading rat embryo cells, α-actinin assumes a focal distribution which corresponds to the vertices of a highly regular actin filament network. The results suggest that α-actinin may be involved in the organization of actin filament bundles, in the attachment of actin filaments to the plasma membrane, and in the assembly of actin filaments in areas of cell to cell contact.
- Date of publication
- 1975
- DOI
- Identifier
- 2-s2.0-0016678780
- https://doi.org/10.1016/0092-8674(75)90180-4
- Related resource URL
- Resource type
- Article
- Rights statement
- In Copyright
- Journal title
- Cell
- Journal volume
- 6
- Journal issue
- 3
- Page start
- 289
- Page end
- 298
- Language
- English
- Version
- Postprint
- ISSN
- 0092-8674
Relations
- Parents:
This work has no parents.
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