Preparing To Use Resonant Acoustic Spectroscopy with Optical Vibrometry to Characterize the Clot Elastic Modulus from Mice with Sickle Cell Disease
Public Deposited- Last Modified
- February 26, 2019
- Creator
-
Segerstrom, Kara J.
- Affiliation: School of Medicine, UNC/NCSU Joint Department of Biomedical Engineering
- Abstract
- CEMport is machine that uses resonant acoustic spectroscopy with optical vibrometry to find the clot elastic modulus. In order to prepare CEMport for future experiments on sickle cell disease, CEMport will be calibrated. The device was previously calibrated to a 150μL cube microwell, but the following study will calibrate CEMport to a 100 μL cylindrical microwell. Microwells with sample sizes 150μL, 125μL and 100μL were tested, and the results demonstrated that all three sizes were able to produce quality fits. The sickle cell blood clots will be formed from mice, therefore the reduction of sample size will allow more samples to be created. The calibration coefficient a[0] in the equation: 𝐸 = 𝑎[0]𝜌𝑓[0]2, was found to be 0.063±0.002 Pa/Hz2, whereas the previously found ratio was 0.076±0.003 Pa/Hz2. Preliminary testing of blood clots for human and mouse whole blood was 1.94±0.03kPa and 2.98±.33kPa respectively, falling within the expected range of elasticity.
- Date of publication
- spring 2017
- Keyword
- DOI
- Resource type
- Rights statement
- In Copyright
- Note
- Funding: None
- Advisor
- Oldenburg, Amy
- Degree
- Bachelor of Science
- Academic concentration
- Biomedical Engineering
- Honors level
- Honors
- Degree granting institution
- University of North Carolina at Chapel Hill
- Graduation year
- 2017
- Language
- English
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