Collections > UNC Chapel Hill Undergraduate Honors Theses Collection > A Novel IgG Modified PEG-PEI Polyplex Formulation for Plasmid Delivery to the Brain
pdf

Ischemic stroke can be associated with varying degrees of neuronal damage and death. To help combat the negative impact of cerebral ischemic events, research has turned its attention to the delivery of plasmids coding for key growth factors to improve neuronal survival. Delivery through the blood brain barrier, however, complicates drug delivery. The use of antibodies presents a unique opportunity to selectively target key receptors on the brain and gain access to this highly selective organ. In this study, a stable formulation for an antibody-conjugated polyplex system was established using polyethylene glycol-polyethylenimine copolymers complexed with plasmid DNA. These polyplexes offer the ability to deliver plasmid DNA through a self-assembling delivery system that has decreased clearance and selective localization to the brain. In establishing a foundation for such polyplexes, this study hopes to demonstrate the utility of this system in drug delivery to the brain.