A method for exposing rodents to resuspended particles using whole-body plethysmography
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Wichers, Lindsay B, et al. A Method for Exposing Rodents to Resuspended Particles Using Whole-body Plethysmography. BioMed Central Ltd, 2006. https://doi.org/10.17615/05cp-6h54APA
Wichers, L., Ledbetter, A., Mc Gee, J., Kellogg, R., Rowan, W., Nolan, J., Costa, D., & Watkinson, W. (2006). A method for exposing rodents to resuspended particles using whole-body plethysmography. BioMed Central Ltd. https://doi.org/10.17615/05cp-6h54Chicago
Wichers, Lindsay B., Allen D Ledbetter, John K Mc Gee, Robert B Kellogg, William H Rowan, Julianne P Nolan, Daniel L Costa et al. 2006. A Method for Exposing Rodents to Resuspended Particles Using Whole-Body Plethysmography. BioMed Central Ltd. https://doi.org/10.17615/05cp-6h54- Creator
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Wichers, Lindsay B.
- Affiliation: Gillings School of Global Public Health, Department of Environmental Sciences and Engineering
- Other Affiliation: National Center for Environmental Assessment
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Ledbetter, Allen D.
- Other Affiliation: US. Environmental Protection Agency
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McGee, John K.
- Other Affiliation: US. Environmental Protection Agency
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Kellogg, Robert B.
- Other Affiliation: Alion Science and Technology Corporation
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Rowan, William H.
- Other Affiliation: US. Environmental Protection Agency
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Nolan, Julianne P.
- Other Affiliation: US. Environmental Protection Agency
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Costa, Daniel L.
- Other Affiliation: U.S. Environmental Protection Agency
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Watkinson, William P.
- Other Affiliation: US. Environmental Protection Agency
- Abstract
- Background Epidemiological studies have reported increased risks of cardiopulmonary-related hospitalization and death in association with exposure to elevated levels of particulate matter (PM) across a wide range of urban areas. In response to these findings, researchers have conducted animal inhalation exposures aimed at reproducing the observed toxicologic effects. However, it is technically difficult to quantitate the actual amount of PM delivered to the lung in such studies, and dose is frequently estimated using default respiration parameters. Consequently, the interpretation of PM-induced effects in rodents exposed via whole-body inhalation is often compromised by the inability to determine deposited dose. To address this problem, we have developed an exposure system that merges the generation of dry, aerosolized particles with whole-body plethysmography (WBP), thus permitting inhalation exposures in the unrestrained rat while simultaneously obtaining data on pulmonary function. Results This system was validated using an oil combustion-derived particle (HP12) at three nominal concentrations (3, 12, and 13 mg/m3) for four consecutive exposure days (6 hr/day); a single 6-hour exposure to 13 mg/m3 of HP12 was also conducted. These results demonstrated that the system was both reliable and consistent over these exposure protocols, achieving average concentrations that were within 10% of the targeted concentration. In-line filters located on the exhaust outlets of individual WBP chambers showed relative agreement in HP12 mass for each day and were not statistically different when compared to one another (p = 0.16). Temperatures and relative humidities were also similar between chambers during PM and air exposures. Finally, detailed composition analyses of both HP12 filter and bulk samples showed that grinding and aerosolization did not change particle chemistry. Conclusion The results of this study demonstrate that it is possible to expose rodents to resuspended, dry PM via whole-body inhalation while these animals are maintained in WBP chambers. This new methodology should significantly improve the ability to assess dosimetry under minimally stressful exposure conditions.
- Date of publication
- August 15, 2006
- DOI
- Identifier
- Resource type
- Article
- Rights statement
- In Copyright
- Rights holder
- Lindsay B Wichers et al.; licensee BioMed Central Ltd.
- License
- Journal title
- Particle and Fibre Toxicology
- Journal volume
- 3
- Journal issue
- 1
- Page start
- 12
- Language
- English
- Is the article or chapter peer-reviewed?
- Yes
- ISSN
- 1743-8977
- Bibliographic citation
- Particle and Fibre Toxicology. 2006 Aug 15;3(1):12
- Publisher
- BioMed Central Ltd
- Access right
- Open Access
- Date uploaded
- September 5, 2012
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