During Wave IV, Add Health collected biological specimens from a large, nationally representative sample of young adults. Given the size of the Wave IV sample, its geographic distribution, and in-home setting of the respondent interviews, biological specimen collection involved practical, relatively non-invasive, cost-efficient and innovative methods. These methods included collection of saliva by trained and certified field interviewers, salivary buccal cell lysis and DNA stabilization in the field, then shipment to a central lab for DNA extraction, genotyping, and archiving. The collection of saliva followed the interview and collection of cardiovascular and anthropometric measures (Entzel et al. 2009). It preceded the collection of capillary whole blood (Whitsel et al. 2012) and data on respondent use of prescription and select over-the-counter medications (Tabor et al. 2010). Further details on the design of Add Health Waves I-IV are available elsewhere (Harris 2012; Harris et al. in press).