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Figure 5 Knockdown of TRIB3 results in lower cell survival under hypoxic conditions. (a) Fold induction of TRIB3 expression levels (mean + standard deviation (SD)) in MDA-MB-231 cells after transfection with negative control siRNA and two siRNAs against TRIB3 transcript. Each measurement was performed in triplicate. (b) Average absorbance at 492 nm (+ SD) measured by MTS assay of cells cultured under normal culture conditions and 0.1% oxygen. Each condition was measured in 10 independent wells.