Monitoring Exercise- and Diet-Induced Changes in Tibial Blood Perfusion with Laser Doppler Flowmetry in Mice [abstract]

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  • Hanne, Nicholas
    • Affiliation: School of Medicine, UNC/NCSU Joint Department of Biomedical Engineering
  • Steward, Andrew
    • Affiliation: School of Medicine, UNC/NCSU Joint Department of Biomedical Engineering
  • Easter, Elizabeth
    • Other Affiliation: Materials Science and Engineering Department, North Carolina State University, Raleigh, NC
  • Cole, Jacqueline
    • Affiliation: School of Medicine, UNC/NCSU Joint Department of Biomedical Engineering
Abstract
  • Vascular function plays a critical role in bone development and repair, and exercise, which stimulates vascular growth and performance [1], may ameliorate detrimental bone changes associated with diabetes or stroke [2]. Measuring changes in bone vasculature in vivo is challenging, and current techniques to monitor functional changes in bone perfusion longitudinally are ineffective. Laser Doppler flowmetry (LDF) can quantify vascular changes in murine tibiae and provide a functional measure of bone perfusion beyond vascular density from histology or growth factor concentrations in serum [3]. However, a previous study reported local inflammation lasting several months, which could confound longitudinal flow measurements [3] and alter normal gait. We performed a less invasive LDF procedure to minimize inflammation and allow longitudinal perfusion measurements. We hypothesized our procedure would detect changes in tibial blood perfusion of young adult male mice with exercise and high fat diet-induced obesity without altering gait.
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  • Conference Proceeding
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  • In Copyright
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  • Proceedings of the IBMS Herbert Fleisch Workshop
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  • Preprint
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  • English

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