A Comparison of Three Dispersion Media on the Physicochemical and Toxicological Behavior of TiO2 and NiO Nanoparticles
Public Deposited- Last Modified
- February 28, 2019
- Creator
-
Gutierrez, Erika Rasche
- Affiliation: Gillings School of Global Public Health, Department of Environmental Sciences and Engineering
- Abstract
- Nanomaterials represent a burgeoning field of technological innovation. With the onset of environmental release associated with nanomaterial manufacture and proliferation, the concomitant effects on human health remain unknown and polemic. Agglomeration of nanomaterials in biologically relevant media further complicates dosing in toxicological study. Objective: To compare the effects of dispersion techniques on the physicochemical and toxicological dosimetry of TiO2(<50nm) and NiO(<20nm) nanoparticles. Methods: Three media were prepared for A549 and 16hbe14o cells with varying concentrations of -TiO2 and NiO nanoparticles. Physicochemical and toxicological effects were analyzed with dynamic light scattering, ICP-MS, SEM, TEM, ELISA, and stimulation of A549 cells with nanoparticles for 4 and 24 hours followed by analysis of inflammatory and oxidative stress markers with RT-PCR. Dispersion media physicochemical properties affect toxicological endpoints. In vitro nanotoxicology models that use re-suspension methods of exposure yield inconsistent biological results due to physicochemical transport processes.
- Date of publication
- May 2013
- DOI
- Resource type
- Rights statement
- In Copyright
- Advisor
- Jaspers, Ilona
- Sexton, Kenneth
- Kamens, R. M.
- Degree
- Master of Science in Public Health
- Academic concentration
- Aquatic and Atmospheric Sciences
- Degree granting institution
- University of North Carolina at Chapel Hill
- Graduation year
- 2013
- Language
- Deposit record
- 5edc2a5d-0dd3-4d26-87fc-ca03080ac860
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