Collections > UNC Scholarly Publications > BioMed Central > Criteria for effective design, construction, and gene knockdown by shRNA vectors > Additional File 2: Gene knockdown is similar for cell lines derived from different rounds of transduction and sorting
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Gene knockdown is similar for cell lines derived from different rounds of transduction and sorting. Realtime data is shown for cell lines derived independently using the same viral vectors. Virus was prepared and used to transduce THP1 cells independently for each round. Values are presented as average + SEM for at least three assays run in duplicate, with the exception of shclr19.3-1504, second transduction (single value).