Characterization of DTACC Structure and Function
Public Deposited- Last Modified
- February 26, 2019
- Creator
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Fadero, Tanner
- Affiliation: College of Arts and Sciences, Department of Biology
- Abstract
- Microtubules (MTs) are essential for numerous cellular processes. MTs impact cell shape, serve as intra-cellular transport tracks, and form the mitotic spindle. Microtubule-associated proteins (MAPs) modulate MTs to afford differing MT dynamics and ultra-structure. The TACC proteins, a highly conserved MAP family, localize to the MT plus-end and to centrosomes where they affect MT dynamics and interact with other MAPs to modulate their MT-regulating potency. We focus on the interplay between TACC and the MT polymerase, XMAP215. To elucidate TACC’s mechanisms of centrosome-localization and XMAP215- recuitment in cells, we used Drosophila S2 cells as a model cell system. We used truncational analysis of Drosophila TACC (DTACC) in order to identify regions of DTACC that localize to the centrosome and to MT plus-ends using fluorescence microscopy. These results suggest the determinants of DTACC that confer in vivo Msps (Drosophila XMAP215) binding and are consistent with our in vitro biochemical binding assays.
- Date of publication
- spring 2015
- Keyword
- DOI
- Resource type
- Rights statement
- In Copyright
- Note
- Funding: None
- Advisor
- Slep, Kevin
- Degree
- Bachelor of Science
- Honors level
- Highest Honors
- Degree granting institution
- University of North Carolina at Chapel Hill
- Extent
- 32
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