Functional analysis of rs3811046 and rs3811047 variants in Caucasians
Public Deposited- Last Modified
- March 22, 2019
- Creator
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Kim, Steven
- Affiliation: School of Dentistry
- Abstract
- Inflammation is an essential for the survival of the host, not only to fight off pathogens but also for immunotolerance to avoid autoimmunity. Turning off the inflammation is as important as turning them on, because lack thereof will lead to uncontrolled chronic inflammation, unnecessary structural damage, and compromised or delayed wound healing which do not benefit the host. Periodontal disease is one of the example of such uncontrolled chronic inflammation. Periodontitis is a combined result of pathogenic bacteria establishing themselves in the biofilm-gingival interface, and the excessive and uncontrolled immune response to the bacteria and their byproducts. It is actually the host immune response that destroys the periodontal attachment apparatus, and not the direct destructive action of the pathogens. Some individuals are more predisposed to inflammation. It can be due to genetic predisposition, others could be due to systemic conditions. We describe one of such example where single nucleotide polymorphisms can lead to pro-inflammatory profiles in Caucasian subjects. In chapter 1, we describe how we honed in onto a number of SNPs that are on the coding region of a cytokine called IL-37. Chapter 2, we created human recombinant IL-37b and observe the effects of variants on its anti-inflammatory function. In chapter 3 we obtained human samples from known genotypes and observed pro-inflammatory profiles in the subjects with the SNP variance. Finally in chapter 4, we discuss its significance and future study directions.
- Date of publication
- August 2017
- Keyword
- DOI
- Resource type
- Rights statement
- In Copyright
- Advisor
- Bencharit, Sompop
- Teles, Ricardo
- Barros, Silvana
- Liu, Zhi
- Offenbacher, Steven
- Degree
- Doctor of Philosophy
- Degree granting institution
- University of North Carolina at Chapel Hill
- Graduation year
- 2017
- Language
Relations
- Parents:
This work has no parents.
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