Statement of problem: Establishing the occlusal plane of the edentulous mouth is challenging but with the accuracy of 3D radiography, the orientation of the occlusal plane can be determined using stable anatomical landmarks. Purpose: The purpose of this study was to determine the location of the occlusal plane in relation to stable, bony anatomical structures. Material and Methods: Stable bony structures were identified and the orientation of the occlusal plane was determined in relation to these landmarks, using CBCT scans. Results: The anterior determinant of occlusal plane is located 29mm (95% CI: 28.3mm to 30.0mm) inferior to the anterior nasal spine (ANS). In relation to the hamular notch-incisal edge (HNI) plane, the occlusal plane forms a 15.5o angle (95% CI: 14.7o to 16.2o). Conclusions: Results from this study will enable dentists and technicians to accurately locate the occlusal plane in a virtual environment without the use of an analog face-bow.