Evaluation of a Sample Concentration Procedure in the Analysis of Dermal Exposure to 1,6-Hexamethylene Diisocyanate and its Oligomers
Public Deposited- Last Modified
- February 28, 2019
- Creator
-
Hodgens, Charles
- Affiliation: Gillings School of Global Public Health, Department of Environmental Sciences and Engineering
- Abstract
- Dermal exposure to 1,6-hexamethylene diisocyanate (HDI) and its oligomers HDI biuret and HDI isocyanurate has been implicated in occupation asthma in automotive spray-painters. Dermal exposure assessment for these compounds has been hampered due to low sample concentrations for these compounds. A sample concentration process, where samples are dried under a stream of nitrogen gas and resuspended in a smaller volume of liquid, was developed and tested in order to improve sensitivity and specificity for sample quantification and exposure assessment. The method was evaluated by comparing tape-strips samples spiked with derivatized standard or clearcoat solutions before and after concentration. In addition, the method was evaluated using 412 dermal tape-strip samples collected from automotive refinishing workers occupationally exposed to diisocyanates. Concentrations of the urea derivatives of HDI, biuret, and isocyanurate were determined using LC-MS analysis via selective ion monitoring. The results showed that both the dry-down process and skin exposure produced significant variation in the spiked sample concentrations. In the worker samples, the dry-down process increased the number of samples with detectable levels of isocyanurate from 125 to 302 while the number of samples for biuret increased from 8 to 70. Interference from unknown compounds impaired analysis of HDI after drying. In summary, the dry-down procedure has a potential to increase the sensitivity and specificity of isocyanurate in the tape-strip samples collected from the exposed workers. However, the potential biases introduced in the sample processing and the increased analytical cost indicate that this method is not suitable in its current state for adaptation to the processing of samples collected from exposed workers.
- Date of publication
- May 2012
- DOI
- Resource type
- Rights statement
- In Copyright
- Advisor
- Ball, Louise
- Thomasen, Jennifer
- Nylander-French, Leena A.
- Degree
- Bachelor of Science in Public Health
- Academic concentration
- Environmental Health Sciences
- Degree granting institution
- University of North Carolina at Chapel Hill
- Graduation year
- 2012
- Language
- Deposit record
- e00f1df6-6c31-41ba-867b-150d86a8c8cb
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