Drosophila Marcal1 in Double-Strand Break Repair by Synthesis Dependent Strand Annealing
Public Deposited- Last Modified
- February 26, 2019
- Creator
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Buzhardt, Emily
- Affiliation: College of Arts and Sciences, Department of Biology
- Abstract
- It is important to study DNA damage such as double strand breaks (DSBs) because DSBs can cause cancer and genomic instability. DSB are repaired by homologous recombination which can cause a crossover or non-crossover event. Crossovers are detrimental because they can lead to genomic instability. Synthesis dependent strand annealing (SDSA) is a form of double strand break repair (DSBR) without crossovers. It is beneficial because it protects against genomic instability. This experiment seeks to determine the role of Marcal1 in SDSA in Drosophila melanogaster using a P{wa} P-element system in a fly line with a Marcal1 deletion. Marcal1 was studied because we have evidence that it is involved in some aspect of DSBR; however, it is unclear about which aspect of DSBR. It is hypothesized that when Marcal1 is deleted and DSBs are induced in the fly line, there will be an increase in aberrant repair. Results show an increase in aberrant repair and a decrease in SDSA, indicating that Marcal1 has a role in SDSA. PCR results of the aberrant repair flies found no statistical difference in the tract lengths between wild type flies and Marcal1 mutant flies.
- Date of publication
- spring 2015
- Keyword
- DOI
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- Rights statement
- In Copyright
- Note
- Funding: None
- Advisor
- Sekelsky, Jeff
- Degree
- Bachelor of Science
- Honors level
- Honors
- Degree granting institution
- University of North Carolina at Chapel Hill
- Extent
- 14
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