Collections > Electronic Theses and Dissertations > Biological Modification of Titanium Surfaces Using Bifunctional Peptides

The clinical success of endosseous implants is associated with the formation and maintenance of bone at implant surfaces. Based on in vivo observations, several generalizations have been derived regarding the nature of the interface. Most prominently, initial cell and molecular adhesion dictate the reactivity of the interface. The aim of this project is to use interfacial biomaterials (Ti-binding peptides) to graft cell adhesive peptides to titanium surfaces used for osseointegrated endosseous implants. We hypothesized that there is statistical significant difference in the differentiation of cells adherent with Ti-binding peptide treated and untreated on titanium surfaces. We used the peptide (AFF6008) can facilitate the adhesion of biologically active peptides to the titanium surface. Scanning electron microscopy was used to quantify cell adhesion on treated and untreated surfaces. Real time polymerase chain reaction was used to measure and compare osteoblast-specific gene expression, ALP and BSP, associated with osteoblastic differentiation in cells cultured on treated and untreated surfaces.