Microbial Transformation of 2,4,6 Trinitrotoluene (TNT) by Cometabolism under Aerobic Conditions
Public Deposited- Last Modified
- February 28, 2019
- Creator
-
Adler, Judy Ann
- Affiliation: Gillings School of Global Public Health, Department of Environmental Sciences and Engineering
- Abstract
- The purpose of this study was to investigate the macro nutrient requirements, toxicity, and kinetics of 2,4,6 trinitrotoluene (TNT) cometabolism by anaerobic microbial consortium derived from TNT-contaminated soil. The results illustrated that succinate and molasses were effective primary substrates for the microbial transformation of TNT. Inorganic nutrient addition was not required for the complete transformation of TNT when molasses and succinate were used as primary substrates. Toxicity (as determined with the Microtox® bioassay) decreased with microbial transformation of TNT but toxicity was not completely eliminated. Kinetic modeling of the cometabolism of TNT with molasses as the primary substrate followed Michaelis-Menten kinetics for the microbial consortium derived from TNT-contaminated soil. Acclimation to TNT was not necessary for the co-metabolism of TNT. A microbial consortium derived from activated sludge that was not acclimated to TNT, transformed TNT at a faster rate than an acclimated consortium derived from TNT contaminated soil.
- Date of publication
- August 1995
- DOI
- Resource type
- Rights statement
- In Copyright
- Advisor
- Pfaender, Frederic K.
- Singer, Philip
- Aitken, Michael
- Degree
- Master of Science in Environmental Engineering
- Academic concentration
- Water Resources Engineering
- Degree granting institution
- University of North Carolina at Chapel Hill
- Graduation year
- 1995
- Language
- Deposit record
- 043fb25a-cea5-4ac2-b0d3-5af4a220fe28
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