Development of a Method for the Isolation and Quantitation of Albumin for Use in the Analysis of Protein Adducts
Public Deposited- Last Modified
- February 28, 2019
- Creator
-
O'Connell, Karen Yeowell
- Affiliation: Gillings School of Global Public Health, Department of Environmental Sciences and Engineering
- Abstract
- In order to improve exposure assessment in epidemiological studies, alternatives to traditional methods such as air sampling are under investigation. The ability to detect individual exposures through the use of biomarkers is thought to be a way to improve the accuracy and specificity of exposure assessment. Protein adducts are biomarkers which show potential for giving more specific information about exposure. Since 1985, investigators have been validating methods that measure adducts of potentially genotoxic species with serum albumin. One of the major difficulties encountered by these researchers is the isolation and quantitation of albumin. This research involved testing two procedures for the isolation of albumin from plasma (affinity chromatography and ammonium sulfate precipitation), two methods for desalting the isolated albumin (gel filtration and dialysis), and two methods for albumin quantitation (Bradford assay and absorbance at 280nm). Based on purity of albumin obtained (as judged by SDS-PAGE), comments in the literature, and applicability of the methods to screening large numbers of plasma samples, a rapid method of albumin isolation and quantitation was developed for use in analyzing protein adducts.
- Date of publication
- December 1992
- DOI
- Resource type
- Rights statement
- In Copyright
- Advisor
- Ball, Louise
- Kamens, R. M.
- Rappaport, Stephen Morris
- Degree
- Master of Science in Public Health
- Academic concentration
- Environmental Health Sciences
- Degree granting institution
- University of North Carolina at Chapel Hill
- Graduation year
- 1992
- Language
- Deposit record
- c2589085-57f7-4434-bf45-a2666ba162b9
Relations
- Parents:
This work has no parents.