Vitamin D receptor expression controls proliferation of naïve CD8+ T cells and development of CD8 mediated gastrointestinal inflammation
Public Deposited- Creator
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Chen, Jing
- Other Affiliation: Department of Veterinary and Biomedical Science, Pathobiology Graduate Program, The Pennsylvania State University
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Bruce, Danny
- Affiliation: School of Medicine
- Other Affiliation: Department of Veterinary and Biomedical Science, Pathobiology Graduate Program, The Pennsylvania State University
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Cantorna, Margherita T
- Other Affiliation: Department of Veterinary and Biomedical Science, Center for Molecular Immunology and Infectious Disease, The Pennsylvania State University
- Abstract
- Abstract Background Vitamin D receptor (VDR) deficiency contributes to the development of experimental inflammatory bowel disease (IBD) in several different models. T cells have been shown to express the VDR, and T cells are targets of vitamin D. In this article we determined the effects of VDR expression on CD8+ T cells. Results VDR KO CD8+ T cells, but not WT CD8+ T cells, induced colitis in Rag KO recipients. In addition, co-transfer of VDR KO CD8+ T cells with naïve CD4+ T cells accelerated colitis development. The more severe colitis was associated with rapidly proliferating naïve VDR KO CD8+ T cells and increased IFN-γ and IL-17 in the gut. VDR KO CD8+ T cells proliferated in vitro without antigen stimulation and did not downregulate CD62L and upregulate CD44 markers following proliferation that normally occurred in WT CD8+ T cells. The increased proliferation of VDR KO CD8+ cells was due in part to the higher production and response of the VDR KO cells to IL-2. Conclusions Our data indicate that expression of the VDR is required to prevent replication of quiescent CD8+ T cells. The inability to signal through the VDR resulted in the generation of pathogenic CD8+ T cells from rapidly proliferating cells that contributed to the development of IBD.
- Date of publication
- February 7, 2014
- DOI
- Identifier
- Resource type
- Article
- Rights statement
- In Copyright
- Rights holder
- Jing Chen et al.; licensee BioMed Central Ltd.
- License
- Journal title
- BMC Immunology
- Journal volume
- 15
- Journal issue
- 1
- Page start
- 6
- Language
- English
- Is the article or chapter peer-reviewed?
- Yes
- ISSN
- 1471-2172
- Bibliographic citation
- BMC Immunology. 2014 Feb 07;15(1):6
- Publisher
- BioMed Central Ltd
- Access right
- Open Access
- Date uploaded
- August 26, 2015
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