Beneficial Use Impairments, Degradation of Aesthetics, and Human Health: A Review
Creator:
Rappazzo, Kristen M., Slawsky, Erik D., Hoffman, Joel C., and Cowan, Kristen N.
Date of publication:
2022
Abstract Tesim:
In environmental programs and blue/green space development, improving aesthetics is a common goal. There is broad interest in understanding the relationship between ecologically sound environments that people find aesthetically pleasing and human health. However, to date, few studies have adequately assessed this relationship, and no summaries or reviews of this line of research exist. Therefore, we undertook a systematic literature review to determine the state of science and identify critical needs to advance the field. Keywords identified from both aesthetics and loss of habitat literature were searched in PubMed and Web of Science databases. After full text screening, 19 studies were included in the review. Most of these studies examined some measure of greenspace/bluespace, primarily proximity. Only one study investigated the impacts of making space quality changes on a health metric. The studies identified for this review continue to support links between green space and various metrics of health, with additional evidence for blue space benefits on health. No studies to date adequately address questions surrounding the beneficial use impairment degradation of aesthetics and how improving either environmental quality (remediation) or ecological health (restoration) efforts have impacted the health of those communities.
Resource type:
Article
Affiliation Label Tesim:
Department of Epidemiology
DOI:
https://doi.org/10.17615/qkbp-4711
Edition:
Publisher
Identifier:
https://dx.doi.org/10.3390/ijerph19106090
ISSN:
1660-4601
Journal Issue:
10
Journal Title:
International Journal of Environmental Research and Public Health
Journal Volume:
19
Keyword:
aesthetic degradation, Great Lakes, green/bluespace, and beneficial use impairments
Language Label:
English
License Label:
Attribution 4.0 International
Other Affiliation:
US EPA
Page Start:
6090
Person:
Rappazzo, Kristen M., Slawsky, Erik D., Hoffman, Joel C., and Cowan, Kristen N.
Increased Ammonium Toxicity in Response to Exogenous Glutamine in Metastatic Breast Cancer Cells
Creator:
Donkin, Shawn S., Sheeley, Madeline P., Kiesel, Violet A., Hursting, Stephen D., Teegarden, Dorothy, and Wendt, Michael K.
Date of publication:
2022
Abstract Tesim:
Several cancers, including breast cancers, show dependence on glutamine metabolism. The purpose of the present study was to determine the mechanistic basis and impact of differential glutamine metabolism in nonmetastatic and metastatic murine mammary cancer cells. Universally labeled 13C5-glutamine metabolic tracing, qRT-PCR, measures of reductive–oxidative balance, and exogenous ammonium chloride treatment were used to assess glutamine reprogramming. Results show that 4 mM media concentration of glutamine, compared with 2 mM, reduced viability only in metastatic cells, and that this decrease in viability was accompanied by increased incorporation of glutamine-derived carbon into the tricarboxylic acid (TCA) cycle. While increased glutamine metabolism in metastatic cells occurred in tandem with a decrease in the reduced/oxidized glutathione ratio, treatment with the antioxidant molecule N-acetylcysteine did not rescue cell viability. However, the viability of metastatic cells was more sensitive to ammonium chloride treatment compared with nonmetastatic cells, suggesting a role of metabolic reprogramming in averting nitrogen cytotoxicity in nonmetastatic cells. Overall, these results demonstrate the ability of nonmetastatic cancer cells to reprogram glutamine metabolism and that this ability may be lost in metastatic cells.
Resource type:
Article
Affiliation Label Tesim:
Department of Nutrition
DOI:
https://doi.org/10.17615/x22r-ah04
Edition:
Publisher
Identifier:
https://dx.doi.org/10.3390/metabo12050469
ISSN:
2218-1989
Journal Issue:
5
Journal Title:
Metabolites
Journal Volume:
12
Keyword:
ammonium toxicity, glutamine metabolism, breast cancer, metastasis, and metabolic reprogramming
Language Label:
English
License Label:
Attribution 4.0 International
Other Affiliation:
Purdue University
Page Start:
469
Person:
Donkin, Shawn S., Sheeley, Madeline P., Kiesel, Violet A., Hursting, Stephen D., Teegarden, Dorothy, and Wendt, Michael K.
Common features of the pericentromere and nucleolus
Creator:
Bloom, K. and Lawrimore, J.
Date of publication:
2019
Abstract Tesim:
Both the pericentromere and the nucleolus have unique characteristics that distinguish them amongst the rest of genome. Looping of pericentromeric DNA, due to structural maintenance of chromosome (SMC) proteins condensin and cohesin, drives its ability to maintain tension during metaphase. Similar loops are formed via condensin and cohesin in nucleolar ribosomal DNA (rDNA). Condensin and cohesin are also concentrated in transfer RNA (tRNA) genes, genes which may be located within the pericentromere as well as tethered to the nucleolus. Replication fork stalling, as well as downstream consequences such as genomic recombination, are characteristic of both the pericentromere and rDNA. Furthermore, emerging evidence suggests that the pericentromere may function as a liquid–liquid phase separated domain, similar to the nucleolus. We therefore propose that the pericentromere and nucleolus, in part due to their enrichment of SMC proteins and others, contain similar domains that drive important cellular activities such as segregation, stability, and repair.
Resource type:
Article
Affiliation Label Tesim:
Department of Biology
DOI:
https://doi.org/10.17615/nj6q-sh61
Edition:
Publisher
Identifier:
https://dx.doi.org/10.3390/genes10121029 and PMID 31835574
ISSN:
2073-4425
Journal Issue:
12
Journal Title:
Genes
Journal Volume:
10
Keyword:
RDNA, Condensin, Cohesin, Pericentromere, and Nucleolus
The NF-κB transcription factor pathway is a crucial regulator of inflammation and immune responses. Additionally, aberrant NF-κB signaling has been identified in many types of cancer. Downstream of key oncogenic pathways, such as RAS, BCR-ABL, and Her2, NF-κB regulates transcription of target genes that promote cell survival and proliferation, inhibit apoptosis, and mediate invasion and metastasis. The cancer stem cell model posits that a subset of tumor cells (cancer stem cells) drive tumor initiation, exhibit resistance to treatment, and promote recurrence and metastasis. This review examines the evidence for a role for NF-κB signaling in cancer stem cell biology.
Resource type:
Article
Affiliation Label Tesim:
UNC Lineberger Comprehensive Cancer Center and Department of Pathology and Laboratory Medicine
DOI:
https://doi.org/10.17615/t3f4-kc54
Edition:
Publisher
Identifier:
https://dx.doi.org/10.3390/cells5020016
ISSN:
2073-4409
Journal Issue:
2
Journal Title:
Cells
Journal Volume:
5
Keyword:
Cancer stem cells, Cancer, Tumor-initiating cells, Self-renewal, and NF-κB
Roles for the IKK-related kinases TBK1 and IKKε in cancer
Creator:
Durand, J.K., Baldwin, A.S., and Zhang, Q.
Date of publication:
2018
Abstract Tesim:
While primarily studied for their roles in innate immune response, the IκB kinase (IKK)-related kinases TANK-binding kinase 1 (TBK1) and IKKε also promote the oncogenic phenotype in a variety of cancers. Additionally, several substrates of these kinases control proliferation, autophagy, cell survival, and cancer immune responses. Here we review the involvement of TBK1 and IKKε in controlling different cancers and in regulating responses to cancer immunotherapy.
Resource type:
Article
Affiliation Label Tesim:
Curriculum in Genetics and Molecular Biology and Department of Pathology and Laboratory Medicine
DOI:
https://doi.org/10.17615/4jh2-dr42
Edition:
Publisher
Identifier:
https://dx.doi.org/10.3390/cells7090139
ISSN:
2073-4409
Journal Issue:
9
Journal Title:
Cells
Journal Volume:
7
Keyword:
Innate immunity, TBK1, Autophagy, Cancer signaling, KRAS, and IKKε
The NF-κB pathway is a critical regulator of immune responses and is often dysregulated in cancer. Two NF-κB pathways have been described to mediate these responses, the canonical and the noncanonical. While understudied compared to the canonical NF-κB pathway, noncanonical NF-κB and its components have been shown to have effects, usually protumorigenic, in many different cancer types. Here, we review noncanonical NF-κB pathways and discuss its important roles in promoting cancer. We also discuss alternative NF-κB-independent functions of some the components of noncanonical NF-κB signaling. Finally, we discuss important crosstalk between canonical and noncanonical signaling, which blurs the two pathways, indicating that understanding the full picture of NF-κB regulation is critical to deciphering how this broad pathway promotes oncogenesis.
Stakeholder perceptions of key aspects of high-quality cancer care to assess with patient reported outcome measures: A systematic review
Creator:
Jansen, J., Stover, A.M., Basch, E.M., Turner, K., Atkinson, T., Carr, P., Walker Bissram, J., Ellis, C.T., Freeman, A.T., and Kurtzman, R.
Date of publication:
2021
Abstract Tesim:
Performance measurement is the process of collecting, analyzing, and reporting standard-ized measures of clinical performance that can be compared across practices to evaluate how well care was provided. We conducted a systematic review to identify stakeholder perceptions of key symptoms and health domains to test as patient-reported performance measures in oncology. Stakeholders included cancer patients, caregivers, clinicians, and healthcare administrators. Standard review methodology was used, consistent with PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses). MEDLINE/PubMed, EMBASE, and the Cochrane Library were searched to identify relevant studies through August 2020. Four coders independently reviewed entries and con-flicts were resolved by a fifth coder. Efficacy and effectiveness studies, and studies focused exclusively on patient experiences of care (e.g., communication skills of providers) were excluded. Searches generated 1813 articles and 1779 were coded as not relevant, leaving 34 international articles for extraction. Patients, caregivers, clinicians, and healthcare administrators prioritize psychosocial care (e.g., distress) and symptom management for patient-reported performance measures. Patients and caregivers also perceive that maintaining physical function and daily activities are critical. Clinicians and administrators perceive control of specific symptoms to be critical (gastrointestinal symptoms, pain, poor sleep). Results were used to inform testing at six US cancer centers.
Resource type:
Article
Affiliation Label Tesim:
UNC Lineberger Comprehensive Cancer Center, Department of Health Policy and Management, University of North Carolina at Chapel Hill. Health Sciences Library, and Department of Health Behavior
DOI:
https://doi.org/10.17615/e1cd-6943
Edition:
Publisher
Identifier:
https://dx.doi.org/10.3390/cancers13143628
ISSN:
2072-6694
Journal Issue:
14
Journal Title:
Cancers
Journal Volume:
13
Keyword:
Patient-centered care, Quality of life, Symptoms, Cancer care delivery, Systematic review, Patient reported outcome measures, Stakeholder perceptions, Quality of healthcare, and High-quality care
Language Label:
English
License Label:
Attribution 4.0 International
Other Affiliation:
Moffitt Cancer Center, Memorial Sloan Kettering Cancer Center, University of Louisville Health, and British Columbia Cancer Agency
Person:
Jansen, J., Stover, A.M., Basch, E.M., Turner, K., Atkinson, T., Carr, P., Walker Bissram, J., Ellis, C.T., Freeman, A.T., and Kurtzman, R.
Exploring a New Theoretical Model to Explain the Behavior of Medication Adherence
Creator:
Unni, Elizabeth and Bae, Sun
Date of publication:
2022
Abstract Tesim:
Medication adherence is essential for optimal therapeutic outcomes. However, non-adherence with long-term therapy is at 50%. Several theoretical models have identified several key factors that could explain medication adherence. Though numerous interventions have been developed based on these theoretical models, the success rates with interventions are not the best. This paper proposes a new Hierarchical Model for Medication Adherence. In this model, we propose medication adherence as a five-tier model with medication adherence as the desirable behavior on the top of the pyramid. From the bottom of the hierarchy upwards, the skills/beliefs/behaviors to be achieved are: health literacy, belief in illness (impacted by perceived susceptibility and severity of illness), belief in medicines (impacted by treatment satisfaction), and self-efficacy (impacted by social support). The model further proposes that each individual will achieve or already have these skills/beliefs/behaviors at various levels. Screening patients for these benchmarks will enable providers to decide where to target interventions.
Resource type:
Article
Affiliation Label Tesim:
Eshelman School of Pharmacy
DOI:
https://doi.org/10.17615/pq2n-yt63
Edition:
Publisher
Identifier:
https://dx.doi.org/10.3390/pharmacy10020043
ISSN:
2226-4787
Journal Issue:
2
Journal Title:
Pharmacy
Journal Volume:
10
Keyword:
medication adherence, hierarchical model, theoretical model, self-efficacy, medication beliefs, health literacy, and illness beliefs
Objectives: To evaluate the feasibility of implementation of an extremity exercise program and to examine its preliminary effects in breast cancer survivors suffering from chemotherapy-induced peripheral neuropathy (CIPN). Sample & Setting: Thirteen breast cancer survivors from one hospital in northern Taiwan. Methods and Variables: A single group with repeated measures, and a quasi-experimental design. The intervention program was a four week, home-based extremity exercise program that was comprised of 10 skilled hand exercises and Buerger-Allen exercises. The Total Neuropathy Scale (clinical version), Functional Assessment of Cancer Therapy/Gynecologic Oncology Group, Neurotoxicity (13-Item Version), Identification Pain Questionnaire, and pain Visual Analogue Scale were used to measure CIPN before exercise (T1), during (T2~T4), and after exercise (T5). Qualitative data were also collected at each time point. Data were analyzed by using descriptive statistics, generalized estimating equations, and directed content analysis. Results: None of the participants reported adverse events during the study period. The extremity exercise program significantly improved patient-reported CIPN after intervention at T4 or T5 but was insignificant on clinician-assessed CIPN. The qualitative data of participant experience indicated that this program is feasible and easy to follow. Conclusion: The extremity exercise program is feasible but needs to increase the sample size and prolong the intervention period for confirmation.
Resource type:
Article
Affiliation Label Tesim:
School of Nursing
DOI:
https://doi.org/10.17615/wkph-1p39
Edition:
Publisher
Identifier:
https://dx.doi.org/10.3390/healthcare10040688
ISSN:
2227-9032
Journal Issue:
4
Journal Title:
Healthcare
Journal Volume:
10
Keyword:
chemotherapy induced peripheral neuropathy (CIPN), Buerger-Allen exercise, ten skilled hand exercise, extremity exercise program, and breast cancer survivors
Language Label:
English
License Label:
Attribution 3.0 United States
ORCID:
Other Affiliation:
National Taiwan University Hospital, , Taipei Veterans General Hospital, China Medical University, China Medical University Hospital, and DaYeh University
Enhancer RNA Transcription Is Essential for a Novel CSF1 Enhancer in Triple-Negative Breast Cancer
Creator:
Kelly, Michael R., Franco, Hector L., Lewis, Michael W., Li, Shen, Regner, Matthew J., Wisniewska, Kamila, King, Caitlin M., and Coffey, Alisha
Date of publication:
2022
Abstract Tesim:
Enhancers are critical regulatory elements in the genome that help orchestrate spatiotemporal patterns of gene expression during development and normal physiology. In cancer, enhancers are often rewired by various genetic and epigenetic mechanisms for the activation of oncogenes that lead to initiation and progression. A key feature of active enhancers is the production of non-coding RNA molecules called enhancer RNAs, whose functions remain unknown but can be used to specify active enhancers de novo. Using a combination of eRNA transcription and chromatin modifications, we have identified a novel enhancer located 30 kb upstream of Colony Stimulating Factor 1 (CSF1). Notably, CSF1 is implicated in the progression of breast cancer, is overexpressed in triple-negative breast cancer (TNBC) cell lines, and its enhancer is primarily active in TNBC patient tumors. Genomic deletion of the enhancer (via CRISPR/Cas9) enabled us to validate this regulatory element as a bona fide enhancer of CSF1 and subsequent cell-based assays revealed profound effects on cancer cell proliferation, colony formation, and migration. Epigenetic silencing of the enhancer via CRISPR-interference assays (dCas9-KRAB) coupled to RNA-sequencing, enabled unbiased identification of additional target genes, such as RSAD2, that are predictive of clinical outcome. Additionally, we repurposed the RNA-guided RNA-targeting CRISPR-Cas13 machinery to specifically degrade the eRNAs transcripts produced at this enhancer to determine the consequences on CSF1 mRNA expression, suggesting a post-transcriptional role for these non-coding transcripts. Finally, we test our eRNA-dependent model of CSF1 enhancer function and demonstrate that our results are extensible to other forms of cancer. Collectively, this work describes a novel enhancer that is active in the TNBC subtype, which is associated with cellular growth, and requires eRNA transcripts for proper enhancer function. These results demonstrate the significant impact of enhancers in cancer biology and highlight their potential as tractable targets for therapeutic intervention.
Resource type:
Article
Affiliation Label Tesim:
UNC Lineberger Comprehensive Cancer Center
DOI:
https://doi.org/10.17615/1dve-4q62
Edition:
Publisher
Identifier:
https://dx.doi.org/10.3390/cancers14071852
ISSN:
2072-6694
Journal Issue:
7
Journal Title:
Cancers
Journal Volume:
14
Keyword:
ovarian cancer, Cas13, eRNA, CRISPR-Cas9, breast cancer, gene expression, dCas9-KRAB, and enhancer
Language Label:
English
License Label:
Attribution 3.0 United States
ORCID:
Other Affiliation:
Page Start:
1852
Person:
Kelly, Michael R., Franco, Hector L., Lewis, Michael W., Li, Shen, Regner, Matthew J., Wisniewska, Kamila, King, Caitlin M., and Coffey, Alisha