Effect of Arsenite and its Metabolite, Methylarsonite, on Glycogen Metabolism in Primary Murine Hepatocytes
Public Deposited- Last Modified
- February 26, 2019
- Creator
-
Fennell, Emily
- Affiliation: Gillings School of Global Public Health, Department of Nutrition
- Abstract
- Environmental inorganic arsenic (iAs) exposure is a major health concern globally. iAs is found in high concentrations in well water, rice products, and other foods. Epidemiological evidence has linked iAs exposure to an increased prevalence and incidence of the diabetic phenotypes, and studies using mouse models have shown that iAs exposure leads to impaired glucose homeostasis, suggesting iAs is a diabetogen. However, the molecular mechanism by which iAs exposure causes diabetes is not well understood. This project focuses on identifying molecular mechanisms by which iAs exposure increases the risk of diabetes. We hypothesize that exposure to iAs or its metabolites disrupts hepatic insulin signaling and impairs the pathways of glycogen metabolism in the liver. Our results demonstrate that exposure to arsenite and methylarsonite, a methylated metabolite of iAs, lead to inhibition of glycogen synthase and activation of glycogen phosphorylase, and reduces glycogen content in primary murine hepatocytes. This data suggests a new mechanism by which iAs exposure may disrupt glucose homeostasis, leading to diabetes.
- Date of publication
- spring 2017
- Keyword
- DOI
- Resource type
- Rights statement
- In Copyright
- Note
- Funding: None
- Advisor
- Styblo, Miroslav
- Degree
- Bachelor of Science in Public Health
- Academic concentration
- Nutrition
- Honors level
- Honors
- Degree granting institution
- University of North Carolina at Chapel Hill
- Graduation year
- 2017
- Language
- English
Relations
- Parents:
This work has no parents.
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